Coding

Part:BBa_K5321008:Design

Designed by: Yufei Zhao   Group: iGEM24_Peking   (2024-09-21)


C-terminal of plum pox virus protease (cPPVp), codon optimized for E. coli, 6x His and FKBP tagged


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Gene synthesis should be performed to obtain this coding sequence.


Source

Nucleotide sequence reconstruction was performed using the amino acid sequences given in the Supplementary Material of Tina Fink et al., 2019, optimized for codon optimization in E. coli.

References